Generator
Part:BBa_K1361004:Design
Designed by: Shoujie Sun Group: iGEM14_Tianjin (2014-10-06)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 764
Illegal NheI site found at 787 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The modifying of CsgA was referring the supplementary of Lu, Michelle Y., et al. "Synthesis and patterning of tunable multiscale materials with engineered cells." (2014).
Source
CsgB and CsgC were collected by PCR from genomic DNA of E coli. DH5alpha strain. CsgA-his was synthesized by GeneScrip.